Toona Sinensis and Moschus Decoction Induced Cell Cycle Arrest in Human Cervical Carcinoma HeLa Cells

Table 1

Effect of the Toona sinensis and Moschus decoction on the mRNA expression of 41 cell cycle related genes. HeLa cells were treated with 50 µg/mL of the decoction for 24 h. The data are presented as the mean ± S.D. (for each group, 𝑛=3).

No.	Symbol	Gene name	Accession no.	Fold change
1	CCNA1	Cyclin A1	NM_003914	0.47*
2	CCNA2	Cyclin A2	NM_001237	1.76
3	CCNB1	Cyclin B1	NM_031966	1.79
4	CCND1	Cyclin D1	NM_053056	0.26*
5	CCND2	Cyclin D2	NM_001759	0.02*
6	CCND3	Cyclin D3	NM_001760	0.96
7	CCNE1	Cyclin E1	NM_057182	0.51
8	CCNE2	Cyclin E2	NM_057749	1.07
9	CCNH	Cyclin H	NM_001239	0.47*
10	CDC25A	Cell division cycle 25 homolog A (S. pombe)	NM_201567	1.64
11	CDC6	Cell division cycle 6 homolog A (S. cerevisiae)	NM_001254	0.91
12	CDC7	Cell division cycle 7 homolog A (S. cerevisiae)	NM_003503	4.09**
13	CDK2	Cyclin-dependent kinase 2	NM_052827	1.01
14	CDK4	Cyclin-dependent kinase 4	NM_000075	1.02
15	CDK6	Cyclin-dependent kinase 6	NM_001259	2.53**
16	CDK7	Cyclin-dependent kinase 7	NM_001799	13.37**
17	CDKN1A	Cyclin-dependent kinase inhibitor 1A (p21, Cip1)	NM_078467	1.15
18	CDKN1B	Cyclin-dependent kinase inhibitor 1B (p27, Kip1)	NM_004064	0.47*
19	CDKN2A	Cyclin-dependent kinase inhibitor 2A	NM_058197	0.88
20	CDKN2B	Cyclin-dependent kinase inhibitor 2B (p15, inhibits CDK4)	NM_078487	0.88
21	CDKN2C	Cyclin-dependent kinase inhibitor 2C (p18, inhibits CDK5)	NM_078626	0.76
22	E2F1	E2F transcription factor 1	NM_005225	0.96
23	E2F2	E2F transcription factor 2	NM_004091	1.17
24	E2F3	E2F transcription factor 3	NM_001949	2.25**
25	HDAC1	Histone deacetylase 1	NM_004964	0.70
26	MCM2	Minichromosome maintenance component 2	NM_004526	0.47*
27	MCM3	Minichromosome maintenance component 3	NM_002388	0.87
28	MCM4	Minichromosome maintenance component 4	NM_182746	1.13
29	MCM5	Minichromosome maintenance component 5	NM_006739	0.92
30	MCM6	Minichromosome maintenance component 6	NM_005915	0.85
31	MCM7	Minichromosome maintenance component 7	NM_182776	1.31
32	ORC1L	Origin recognition complex, subunit 1-like (yeast)	NM_004153	0.94
33	ORC2L	Origin recognition complex, subunit 2-like (yeast)	NM_006190	1.24
34	ORC6L	Origin recognition complex, subunit 6-like (yeast)	NM_014321	0.42*
35	PCNA	Proliferation cell nuclear antigen	NM_182649	0.87
36	PKMYT1	Protein kinase, membrane associated tyrosine/threonine 1	NM_182687	1.13
37	RB1	Retinoblastoma 1	NM_000321	2.30**
38	RBL1	Retinoblastoma-like 1 (p107)	NM_183404	1.18
39	SKP2	S-Phase kinase-associated protein 2, E3 ubiquitin protein ligase	NM_032637	1.67
40	TFDP1	Transcription factor Dp-1	NM_007111	1.28
41	WEE1	WEE 1 homolog (S. pombe)	NM_003390	0.47*
42^#	B2M	Beta-2-microglobulin	NM_004048
43^#	ACTB	Beta actin	NM_001101
44^#	GAPDH	Glyceraldehyde-3-phosphate dehydrogenase	NM_002046
45^#	HPRT1	Hypoxanthine phosphoribosyltransferase 1	NM_000194
46^#	OAZ1	Ornithine decarboxylase antizyme 1	NM_004152

#The B2M, ACTB, GAPDH, HPRT1, and OAZ1 were selected as internal controls.
*Ratio < 0.5. **Ratio > 2.
**Means that “ratio > 2”.