Effects of Chinese Medicine Tong xinluo on Diabetic Nephropathy via Inhibiting TGF-β1-Induced Epithelial-to-Mesenchymal Transition
Figure 1
Effects of TXL on the expressions of TGF-β1, E-CA, and α-SMA in renal tissues of KK-Ay mice. (a) mRNA expression of TGF-β1 was determined by RT-PCR with β-actin as an internal control. (b) mRNA expressions of E-CA and α-SMA were determined by RT-PCR with β-actin as an internal control. (c) Representative bands of TGF-β1, E-CA, and α-SMA detected by western blot. (d) Representative immunofluorescence staining photographs of E-CA and α-SMA, visualized by confocal microscope. Images are shown at 20x. (e) Mean fluorescence activity of E-CA and α-SMA analyzed by image-pro plus 6.0 software. (f) Densitometry analysis of TGF-β1, E-CA, and α-SMA bands from (c), normalized to β-actin.
