Figure 3: Flow cytometry analysis of GS-Rb1 on intracellular ROS induced by H/I. (a) NRCMs were cotreated with or without GS-Rb1 (10, 20, and 40 μM) during H/I for 24 h. The intracellular ROS level was measured by the fluorescent probe DCFH-DA. (b) Analysis of the mean fluorescence intensity of each group. Error bars represent . ∗∗∗ versus control, # , ## , and ### versus H/I group ( ).