Figure 4: Flow cytometry analysis of GS-Rb1 on mitochondrial membrane potential ( m) induced by H/I. (a) NRCMs were cotreated with or without GS-Rb1 (10, 20, and 40 μM) during H/I for 24 h. m was measured by JC-1 and analyzed by flow cytometry. (b) Analysis of the red/green fluorescence ratio of each group. Error bars represent mean ± SD. ∗∗∗ versus control, # , ## , and ### versus H/I group, ( ).