Table of Contents Author Guidelines Submit a Manuscript
Evidence-Based Complementary and Alternative Medicine
Volume 2014, Article ID 149195, 10 pages
http://dx.doi.org/10.1155/2014/149195
Research Article

Ginsenoside Rb1 Protects Neonatal Rat Cardiomyocytes from Hypoxia/Ischemia Induced Apoptosis and Inhibits Activation of the Mitochondrial Apoptotic Pathway

1Beijing Haidian Hospital (Haidian Section of Peking University Third Hospital), 29 Zhongguancun Dajie, Haidian District, Beijing 100080, China
2Postdoctoral Workstation of the Zhongguancun Haidian Science Park, NO. 6, Sijiqing Road, Haidian District, Beijing 100195, China
3Institute of Geriatric Cardiology, The General Hospital of People’s Liberation Army, 28 Fuxing Road, Beijing 100853, China
4Beijing Hospital of Integrated Traditional Chinese and Western Medicine, Dongjie 3, Yongding Road, Haidian District, Beijing 100039, China
5Beijing Institute of Radiation Medicine, Beijing 100850, China

Received 1 April 2014; Revised 2 June 2014; Accepted 23 June 2014; Published 10 July 2014

Academic Editor: Ke-Ji Chen

Copyright © 2014 Xu Yan et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. V. G. Sharov, A. Todor, S. Khanal, M. Imai, and H. N. Sabbah, “Cyclosporine A attenuates mitochondrial permeability transition and improves mitochondrial respiratory function in cardiomyocytes isolated from dogs with heart failure,” Journal of Molecular and Cellular Cardiology, vol. 42, no. 1, pp. 150–158, 2007. View at Publisher · View at Google Scholar · View at Scopus
  2. Q. Li, L. Zhou, G. Gao, J. Jiao, and P. Li, “Mitochondrial network in the heart,” Protein and Cell, vol. 3, no. 6, pp. 410–418, 2012. View at Publisher · View at Google Scholar · View at Scopus
  3. L. Bouchier-Hayes, L. Lartigue, and D. D. Newmeyer, “Mitochondria: pharmacological manipulation of cell death,” The Journal of Clinical Investigation, vol. 115, no. 10, pp. 2640–2647, 2005. View at Publisher · View at Google Scholar · View at Scopus
  4. C. M. Schonhoff, B. Gaston, and J. B. Mannick, “Nitrosylation of cytochrome c during apoptosis,” Journal of Biological Chemistry, vol. 278, no. 20, pp. 18265–18270, 2003. View at Publisher · View at Google Scholar · View at Scopus
  5. C. Guo, X. Zeng, J. Song et al., “A soluble receptor for advanced glycation end-products inhibits hypoxia/reoxygenation-induced apoptosis in rat cardiomyocytes via the mitochondrial pathway,” International Journal of Molecular Sciences, vol. 13, no. 9, pp. 11923–11940, 2012. View at Publisher · View at Google Scholar · View at Scopus
  6. H. Huang, K. Wu, Q. You, R. Huang, and S. Li, “Naringin inhibits high glucose-induced cardiomyocyte apoptosis by attenuating mitochondrial dysfunction and modulating the activation of the p38 signaling pathway,” International Journal of Molecular Medicine, vol. 32, no. 2, pp. 396–402, 2013. View at Publisher · View at Google Scholar · View at Scopus
  7. M. L. Xu, H.-J. Kim, Y.-R. Choi, and H.-J. Kim, “Intake of Korean red ginseng extract and saponin enhances the protection conferred by vaccination with inactivated influenza A virus,” Journal of Ginseng Research, vol. 36, no. 4, pp. 396–402, 2012. View at Publisher · View at Google Scholar · View at Scopus
  8. L. Jia, Y. Zhao, and X. Liang, “Current evaluation of the millennium phytomedicine - Ginseng (II): Collected chemical entities, modern pharmacology, and clinical applications emanated from traditional chinese medicine,” Current Medicinal Chemistry, vol. 16, no. 22, pp. 2924–2942, 2009. View at Publisher · View at Google Scholar · View at Scopus
  9. J. Li, Z. H. Shao, J. Z. Xie et al., “The effects of ginsenoside Rb1 on JNK in oxidative injury in cardiomyocytes,” Archives of Pharmacal Research, vol. 35, no. 7, pp. 1259–1267, 2012. View at Publisher · View at Google Scholar · View at Scopus
  10. Y. Wu, Z. Y. Xia, J. J. Dou et al., “Protective effect of ginsenoside Rb1 against myocardial ischemia/reperfusion injury in streptozotocin-induced diabetic rats,” Molecular Biology Reports, vol. 38, no. 7, pp. 4327–4335, 2011. View at Publisher · View at Google Scholar · View at Scopus
  11. R. Xia, B. Zhao, Y. Wu et al., “Ginsenoside Rb1 preconditioning enhances eNOS expression and attenuates myocardial ischemia/reperfusion injury in diabetic rats,” Journal of Biomedicine and Biotechnology, vol. 2011, Article ID 767930, 8 pages, 2011. View at Publisher · View at Google Scholar · View at Scopus
  12. P. Simpson and S. Savion, “Differentiation of rat myocytes in single cell cultures with and without proliferating nonmyocardial cells. Cross-striations, ultrastructure, and chronotropic response to isoproterenol,” Circulation Research, vol. 50, no. 1, pp. 101–116, 1982. View at Publisher · View at Google Scholar · View at Scopus
  13. S. Tan, F. Zhou, and N. Li, “Anti-fatigue effect of ginsenoside Rb1 on postoperative fatigue syndrome induced by major small intestinal resection in rat,” Biological & Pharmaceutical Bulletin, vol. 36, pp. 1634–1639, 2013. View at Google Scholar
  14. L. Shen, Y. Xiong, D. Q. Wang et al., “Ginsenoside Rb1 reduces fatty liver by activating AMP-activated protein kinase in obese rats,” Journal of Lipid Research, vol. 54, no. 5, pp. 1430–1438, 2013. View at Publisher · View at Google Scholar · View at Scopus
  15. N. Lin, D. Cai, D. Jin, Y. Chen, and J. Shi, “Ginseng panaxoside Rb1 reduces body weight in diet-induced obese mice,” Cell Biochemistry and Biophysics, vol. 68, no. 1, pp. 189–194, 2014. View at Publisher · View at Google Scholar · View at Scopus
  16. T. H. Kang, H. M. Park, Y. Kim et al., “Effects of red ginseng extract on UVB irradiation-induced skin aging in hairless mice,” Journal of Ethnopharmacology, vol. 123, no. 3, pp. 446–451, 2009. View at Publisher · View at Google Scholar · View at Scopus
  17. D. Liu, H. Zhang, W. Gu, Y. Liu, and M. Zhang, “Neuroprotective effects of ginsenoside rb1 on high glucose-induced neurotoxicity in primary cultured rat hippocampal neurons,” PLoS ONE, vol. 8, no. 11, Article ID e79399, 2013. View at Publisher · View at Google Scholar
  18. Z. Liu, J. Chen, W. Huang, Z. Zeng, Y. Yang, and B. Zhu, “Ginsenoside Rb1 protects rat retinal ganglion cells against hypoxia and oxidative stress,” Molecular Medicine Reports, vol. 8, pp. 1397–1403, 2013. View at Google Scholar
  19. J. Wang, L. Qiao, S. Li, and G. Yang, “Protective effect of ginsenoside rb1 against lung injury induced by intestinal ischemia-reperfusion in rats,” Molecules, vol. 18, no. 1, pp. 1214–1226, 2013. View at Publisher · View at Google Scholar · View at Scopus
  20. J. Wang, L. Qiao, Y. Li, and G. Yang, “Ginsenoside Rb1 attenuates intestinal ischemia-reperfusion-induced liver injury by inhibiting NF-κB activation,” Experimental and Molecular Medicine, vol. 40, no. 6, pp. 686–698, 2008. View at Publisher · View at Google Scholar · View at Scopus
  21. Q. Sun, Q. Meng, Y. Jiang, and Z. Xia, “Ginsenoside Rb1 attenuates intestinal ischemia reperfusion induced renal injury by activating Nrf2/ARE pathway,” Molecules, vol. 17, no. 6, pp. 7195–7205, 2012. View at Publisher · View at Google Scholar · View at Scopus
  22. Y. Lee and Å. B. Gustafsson, “Role of apoptosis in cardiovascular disease,” Apoptosis, vol. 14, no. 4, pp. 536–548, 2009. View at Publisher · View at Google Scholar · View at Scopus
  23. J. Song, X. Teng, Y. Cai, C. Tang, and Y. Qi, “Activation of Akt/GSK-3β signaling pathway is involved in intermedin1–53 protection against myocardial apoptosis induced by ischemia/reperfusion,” Apoptosis, vol. 14, no. 11, pp. 1299–1307, 2009. View at Publisher · View at Google Scholar · View at Scopus
  24. S. Cadenas, J. Aragonés, and M. O. Landázuri, “Mitochondrial reprogramming through cardiac oxygen sensors in ischaemic heart disease,” Cardiovascular Research, vol. 88, no. 2, pp. 219–228, 2010. View at Publisher · View at Google Scholar · View at Scopus
  25. J. D. Ly, D. R. Grubb, and A. Lawen, “The mitochondrial membrane potential (δψm) in apoptosis; an update,” Apoptosis, vol. 8, no. 2, pp. 115–128, 2003. View at Publisher · View at Google Scholar · View at Scopus
  26. D. B. Zorov, M. Juhaszova, and S. J. Sollott, “Mitochondrial ROS-induced ROS release: an update and review,” Biochimica et Biophysica Acta, vol. 1757, no. 5-6, pp. 509–517, 2006. View at Publisher · View at Google Scholar · View at Scopus
  27. M. L. Circu and T. Y. Aw, “Reactive oxygen species, cellular redox systems, and apoptosis,” Free Radical Biology and Medicine, vol. 48, no. 6, pp. 749–762, 2010. View at Publisher · View at Google Scholar · View at Scopus
  28. H. L. Kong, Z. Q. Li, Y. J. Zhao et al., “Ginsenoside Rb1 protects cardiomyocytes against CoCl2-induced apoptosis in neonatal rats by inhibiting mitochondria permeability transition pore opening,” Acta Pharmacologica Sinica, vol. 31, no. 6, pp. 687–695, 2010. View at Publisher · View at Google Scholar · View at Scopus
  29. E. Yang and S. J. Korsmeyer, “Molecular thanatopsis: a discourse on the BCL2 family and cell death,” Blood, vol. 88, no. 2, pp. 386–401, 1996. View at Google Scholar · View at Scopus
  30. G. van Loo, X. Saelens, M. van Gurp, M. MacFarlane, S. J. Martin, and P. Vandenabeele, “The role of mitochondrial factors in apoptosis: a Russian roulette with more than one bullet,” Cell Death & Differentiation, vol. 9, no. 10, pp. 1031–1042, 2002. View at Publisher · View at Google Scholar · View at Scopus
  31. H. M. Honda and P. Ping, “Mitochondrial permeability transition in cardiac cell injury and death,” Cardiovascular Drugs and Therapy, vol. 20, no. 6, pp. 425–432, 2006. View at Publisher · View at Google Scholar · View at Scopus
  32. H. Yuanming, M. A. Benedict, L. Ding, and G. Núñez, “Role of cytochrome c and dATP/ATP hydrolysis in Apaf-1-mediated caspase-9 activation and apoptosis,” EMBO Journal, vol. 18, no. 13, pp. 3586–3595, 1999. View at Publisher · View at Google Scholar · View at Scopus
  33. K. Nishida, O. Yamaguchi, and K. Otsu, “Crosstalk between autophagy and apoptosis in heart disease,” Circulation Research, vol. 103, no. 4, pp. 343–351, 2008. View at Publisher · View at Google Scholar · View at Scopus