Neuroprotective Effects of Cuscutae Semen in a Mouse Model of Parkinson’s Disease

Figure 7

MPTP-induced ROS generation is inhibited by CS. CS inhibits MPTP-induced and -derived oxidant production and protein oxidation in the SN. The animals received hydroethidine 48 h after the intranigral injection of MPTP in the absence or presence of CS. After 45 min, the brains were harvested, and sections of the SN were prepared for hydroethidine histochemistry to detect extracellular superoxide. Confocal microscopic observation revealed ethidium fluorescence (red) in the SN. The nuclei were counterstained with Hoechst 33258 (blue). These data are representative of five to six animals per group. Dotted lines indicate the SN. The schematic brain sections are from the atlas (Paxinos and Watson, 1986). The square represents SN in the mouse brain.