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Evidence-Based Complementary and Alternative Medicine
Volume 2014, Article ID 150617, 9 pages
Research Article

Mulberry Extracts Alleviate Aβ25–35-Induced Injury and Change the Gene Expression Profile in PC12 Cells

1Department of Nutrition, Tianjin Institute of Health and Environmental Medicine, Tianjin 300050, China
2Comparative Medicine Center, Institute of Laboratory Animal Science, Peking Union Medical College (PUMC) and Chinese Academy of Medical Sciences (CAMS), Beijing 100021, China
3Tianjin Agricultural College, 22 Jinjing Highway, Tianjin 300384, China
4Center for Disease Control and Prevention, PLA Chengdu Military Area Command, Chengdu 610021, China
5Department of Preventive Medicine, Hangzhou Normal University, Hangzhou 311121, China

Received 9 July 2014; Revised 27 October 2014; Accepted 28 October 2014; Published 17 December 2014

Academic Editor: Cheorl-Ho Kim

Copyright © 2014 Nan Song et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Mulberry fruit extracts (ME) contain high amounts of anthocyanins. The supplementary material involved in the preparation processes of mulberry extracts and determination of total anthocyanins content. Mulberries and their major neuroprotective compound—C3G (cyanidin-3-O-β-D-glucopyranoside) have demonstrated the neuroprotective effect on a cerebral infarction in mouse brain injury model and H2O2-induce oxidative damage in PC12 cells. Yet few studies have used mulberry fruit extracts (mixture) as the only intervention substance to investigate cytoprotective and neuroprotective effects on Aβ25-35-induced injury model in PC12 cells. So this supplementary material also involved in the morphological shapes of PC12 cells induced by NGF and the morphological shapes of differentiated PC12 cells in different treatment groups. To explore mechanisms involved, we use the genomic techniques to quickly and accurately quantify vast numbers of potential gene expressions. Table 5 and 6 in supplementary material illustrated the significantly changed genes in PC12 cells after different treatment.

  1. Supplementary Material