Screening for Bioactive Metabolites in Plant Extracts Modulating Glucose Uptake and Fat Accumulation
Figure 1
Effect of DCM extracts of golden root (Rhodiola rosea) and thyme (Thymus vulgaris) on PPARγ transactivation (a) and glucose uptake (GU) in adipocytes (b). DMSO (vehicle, set to 1.00) and results were normalized to this. For PPARγ, positive control was rosiglitazone (Rosi, fold activation 79.7 relative to DMSO) and test concentrations of extracts were 1, 10, and 100 μg/mL (a). The GU test was performed at different concentrations of insulin (0, 3, 10, 30, and 100 nM), while the extract concentration was kept at 100 μg/mL (b). Plotted values are least square means ± SD of mean of 3 replicates. , , and indicate significance relative to DMSO.
