Screening for Bioactive Metabolites in Plant Extracts Modulating Glucose Uptake and Fat Accumulation
Figure 3
Effect of DCM extracts of golden root (Rhodiola rosea) and thyme (Thymus vulgaris) on fat accumulation in C. elegans. Activation by DMSO (vehicle set to 1.00) and results are given as fluorescence levels relative to this (a). All extracts were tested at 200 μg/mL. Lipid stores in C. elegans were stained with nile red. Differential interference microscopy and nile red images (rhodamine channel) of L4 staged wild-type worms treated with either vehicle (b and c) or 200 µg/mL DCM extract of thyme (d and e) are shown. Fluorescence levels are shown as normalized means ± normalized SEM. , , and indicate significance relative to DMSO.
