Research Article

Chemical Assessment and Antimicrobial and Antioxidant Activities of Endophytic Fungi Extracts Isolated from Costus spiralis (Jacq.) Roscoe (Costaceae)

Table 4

TLC results of secondary metabolites in the fungal extracts.

Crude extract code Endophytic speciesUV 365 nm1NEU2 Chloridric vanillin3KOH 10%4KOH 10%5 Dragendorf6Sulfuric vanillin7Sulfuric vanillin8Liebermann-Burchard9Liebermann-Burchard10

CEP1 Phomopsis sp.(+)(+)(−)(+)(−)(−)(+)(−)(+)(+)
CEDp2 Diaporthe phaseolorum (+)(+)(−)(+)(−)(−)(−)(−)(+)(+)
CED3 Diaporthe sp.(+)(+)(−)(+)(−)(−)(+)(−)(+)(+)
CED4 Diaporthe sp.(+)(+)(−)(+)(−)(−)(+)(−)(−)(+)
CEP4 Phomopsis sp.(+)(+)(−)(+)(−)(−)(+)(−)(−)(+)
CE6 Not identified(+)(+)(−)(+)(−)(−)(+)(+)(+)(+)
CED7 Diaporthe sp.(+)(+)(−)(+)(−)(−)(+)(−)(−)(+)
CES8 Sordariomycetes sp.(+)(+)(−)(+)(−)(−)(+)(−)(−)(+)
CE9 Not identified(+)(+)(−)(+)(−)(−)(+)(−)(+)(+)
CEP10 Phomopsis sp.(+)(+)(−)(+)(−)(−)(+)(−)(−)(+)
CEDp11 Diaporthe phaseolorum (+)(+)(−)(+)(+)(−)(+)(−)(−)(+)
CEC12 Cochliobolus ssp.(+)(+)(−)(+)(−)(−)(+)(−)(−)(+)
CES13 Sordariomycetes sp.(+)(+)(−)(+)(−)(−)(+)(−)(−)(+)
CECn (−)(−)(−)(−)(−)(−)(−)(−)(−)(−)

(+): positive; (−): negative; CECn: crude extract control negative.
1Coumarins; 2phenolic compounds; 3protoanthocyanins, leucoanthocyanins; 4coumarins (mobile phase to coumarins); 5anthraquinones, naphthoquinone, and anthocyanins (mobile phase to anthraquinones); 6alkaloids; 7phenols, sterols, terpenes, higher alcohols, polyketides, and saponins (mobile phase to saponins); 8phenols, sterols, terpenes, higher alcohols, polyketides, and saponins (mobile phase to mono-, sesqui-, and diterpenes); 9triterpenes (pink-reddish band); 10sterols (gray band).