Akebia trifoliate (Thunb.) Koidz Seed Extract Inhibits the Proliferation of Human Hepatocellular Carcinoma Cell Lines via Inducing Endoplasmic Reticulum Stress

Figure 3

Different cell cycle influences of ATSE on HCC cell lines. Cell cycle analysis was performed by Flow Cytometry. G418 (0.45 mg/mL); ATSE: Akebia trifoliate seed extract (0.625 mg/mL); 1/2(G418 + ATSE): a combination of half dosage of each accordingly. (a–d) Images of HepG2 cells after different treatments, (e–h) images of HuH7 cells after different treatments, (i–l) images of SMMC-7721 cells after different treatments, (m) HepG2 cell cycle distribution, (n) HuH7 cell cycle distribution, and (o) SMMC-7721 cell cycle distribution. M1: G0/G1 phase, M2: G2/M phase, and M3: S phase. continuous peak after G2/M phase after ATSE treatment, apoptotic body peak, and apoptotic cell peak. The results are expressed as mean ± S.D. . and (versus control group); and (versus G418 group).

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