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Evidence-Based Complementary and Alternative Medicine
Volume 2014 (2014), Article ID 195305, 11 pages
Research Article

Wild Mushrooms in Nepal: Some Potential Candidates as Antioxidant and ACE-Inhibition Sources

1Faculty of Agriculture, Kyushu University, Fukuoka 812-8581, Japan
2Department of Chemistry, Hue College of Sciences, Hue University, 77 Nguyen Hue, Hue, Vietnam
3Miyazaki Prefectural Wood Utilization Research Center, Miyazaki 885-0037, Japan
4International College of Arts and Sciences, Fukuoka Women's University, Fukuoka 813-8529, Japan
5Material Management Center, Kyushu University, Fukuoka 812-8581, Japan
6Plant Pathology Division, Nepal Agriculture Research Council, Khumaltar, Lalitpur, Nepal, P.O. Box. 3605, Kathmandu, Nepal
7Graduate School of Systems Life Sciences, Kyushu University, Fukuoka 812-8581, Japan
8254 Adhikari Niwas, Alka Basti, Lainchour, P.O. Box 21758, 29, Kathmandu, Nepal

Received 1 May 2013; Accepted 4 December 2013; Published 28 January 2014

Academic Editor: Yoshiji Ohta

Copyright © 2014 Tran Hai Bang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Twenty-nine mushrooms collected in the mountainous areas of Nepal were analyzed for antioxidant activity by different methods, including Folin-Ciocalteu, ORAC, ABTS, and DPPH assays. Intracellular H2O2-scavenging activity was also performed on HaCaT cells. The results showed that phenolic compounds are the main antioxidant of the mushrooms. Among studied samples, Inonotus andersonii, and Phellinus gilvus exhibited very high antioxidant activity with the phenolic contents up to 310.8 and 258.7 mg GAE/g extracts, respectively. The H2O2-scavenging assay on cells also revealed the potential of these mushrooms in the prevention of oxidative stress. In term of ACE-inhibition, results showed that Phlebia tremellosa would be a novel and promising candidate for antihypertensive studies. This mushroom exhibited even higher in vitro ACE-inhibition activity than Ganoderma lingzhi, with the IC50 values of the two mushrooms being 32 μg/mL and 2 μg/mL, respectively. This is the first time biological activities of mushrooms collected in Nepal were reported. Information from this study should be a valuable reference for future studies on antioxidant and ACE-inhibitory activities of mushrooms.