Diarylheptanoids from Alpinia officinarum Cause Distinct but Overlapping Effects on the Translatome of B Lymphoblastoid Cells
Figure 3
Scatter plot of normalized intensities versus control intensities (a) and of normalized intensities of genes affected by each diarylheptanoid (b). (a) Net intensity for each spot: the net intensity was calculated by subtracting surrounding background intensity, which was derived from row intensity, from the intensity of the respective spot. Normalization was performed by adjusting the median of all the read microarray data relative to a fixed value. After the net intensity of each probe was normalized, a sample versus control ratio was calculated. For those probes with 2-fold upregulation (Ratio 2) or downregulation (Ratio 0.5) in treated cells and that exhibited a normalized net intensity of more than 40, the net intensity values were plotted. (b) Normalized net intensity values of genes that were regulated by each diarylheptanoid were potted versus control normalized net intensity values.
