Evidence-Based Complementary and Alternative Medicine

Research Article

Diarylheptanoids from Alpinia officinarum Cause Distinct but Overlapping Effects on the Translatome of B Lymphoblastoid Cells

Table 2

Number of WikiPathways transcripts that exhibited altered polysomal loading following AO-0001, AO-0002, or AO-0003.


WikiPathways	Total gene no.	AO-0001		AO-0002		AO-0003
Pathway name	Total gene no.	score	Altered gene number	score	Altered gene number	score	Altered gene number

Alanine and aspartate metabolism WP106 41117	12	2.73	4			3.33	5
Apoptosis WP254 41184	79	−2.18	2			−2.45	2
Calcium regulation in the cardiac cell WP536 41204	145					−2.19	8
Cell cycle WP179 45137	72					2.93	16
Diurnally regulated genes with circadian orthologs WP410 41104	42	2.52	9
DNA replication WP466 41036^*1	38	3.95	11				13
Glycolysis and gluconeogenesis WP534 41077	45	3.29	11				14
GPCRs, class A rhodopsin-like WP455 41121^*2	227	−3.40	7	−2.67	13	−3.05	11
MAPK signaling pathway WP382 41048^*3	153					−2.10	9
mRNA processing WP411 45374^*4	110	7.40	34	5.54	31	9.80	45
Proteasome degradation WP183 45274	52			3.09	13
Translation factors WP107 41026	42			3.02	11	3.05	11

WikiPathways transcripts with a value < 0.5 are listed.
^*1DNA: deoxyribonucleic acids
^*2GPCRs: G protein-coupled receptors
^*3MAPK: mitogen-activated protein kinases
^*4The following 16 transcripts were each upregulated after treatment with each diarylheptanoid (AO-0001, AO-0002, or AO-0003). CSTF1: cleavage stimulation factor, 3′ pre-RNA, subunit 1, 50 kDa; CSTF3: cleavage stimulation factor, 3′ pre-RNA, subunit 3, 77 kDa; DDX1: DEAD (Asp-Glu-Ala-Asp) box helicase 1; FUS: fused in sarcoma; HNRNPAB: heterogeneous nuclear ribonucleoprotein A/B; HNRNPC: heterogeneous nuclear ribonucleoprotein C (C1/C2); HNRNPK: heterogeneous nuclear ribonucleoprotein K; NONO: non-POU domain containing, octamer-binding; PTBP1: polypyrimidine tract binding protein 1; PRPF40A: PRP40 pre-mRNA processing factor 40 homolog A (S. cerevisiae); PRPF6: PRP6 pre-mRNA processing factor 6 homolog (S. cerevisiae); SRSF7: serine/arginine-rich splicing factor 7; SNRNP70: small nuclear ribonucleoprotein 70 kDa (U1); SF3A3: splicing factor 3a, subunit 3, 60 kDa; SF3B2: splicing factor 3b, subunit 2, 145 kDa; U2AF2: U2 small nuclear RNA auxiliary factor 2.