Evidence-Based Complementary and Alternative Medicine / 2014 / Article / Tab 1

Research Article

Molecular Mechanism of Yisui Shengxue Granule, a Complex Chinese Medicine, on Thalassemia Patients Suffering from Hemolysis and Anemia of Erythrocytes

Table 1

Primer used for detecting the gene expression by reverse real-time PCR.

Target genePrimer sequence 5′-3′Predicted amplification segments (bp)
Forward primerReverse primer

β-Actin GAG ACC TTC AAC ACC CCA GCCAAT GTC ACG CAC GAT TTC CC263
SPTA1 GAT CTT GAA GCC AAT GTC CACAA CTC CCT CCA CTG GTG250
SPTB AAC CGG GAT AAG GTC TTG AGT CGGT GCT TTA GCC ATT TAT TGT GA289
EPB4.1 TTA TCC ACT CAC TCA CCC TTC CCTC ATC AGC AAT CTC GGT CTC C369
BCL11A CGC CAG AGG ATG ACG ATCAG GCG TGG GGA TTA GA138
α-GlobinCCC ACC ACC AAG ACC TAC TTGCT CAC AGA AGC CAG GAA CTT291
β-GlobinTAG CAA CCT CAA ACA GAC ACC A ATC ACT AAA GGC ACC GAC CAC T243
Aγ-GlobinATC AAT AAG CTC CTA GTC CAG ACG CCA AAG CTG TCA AAG AAC CTC T166
Gγ-Globin AGG AGG ACA AGG CTA CTA TCA CAACTT GAG ATC ATC CAG GTG CTT TAT233
DNMT1TAC CTG GAC GAC CCT GAC CTCCGT TGG CAT CAA AGA TGG ACA 103
DNMT3aTAT TGA TGA GCG CAC AAG AGA GCGGG TGT TCC AGG GTA ACA TTG AG111
DNMT3bGGC AAG TTC TCC GAG GTC TCT GTGG TAC ATG GCT TTT CGA TAG GA 132