Effect of Nourishing “Yin”-Removing “Fire” Chinese Herbal Mixture on Hypothalamic NKB/NK3R Expression in Female Precocious Rats
Figure 3
Effect of Chinese herb mixture on hypothalamic NKB/NK3R expression by immunofluorescence. (a) Representative microscopic images showing that the NKBir positive neurons located in ARC on the day of onset-puberty decreased in CHM rats compared with M ones (low magnification 10x, high magnification 40x). (b) Representative microscopic images showing that the NK3Rir positive neurons located in ARC on the day of onset-puberty decreased in CHM rats compared to M (low magnification 10x, high magnification 40x). (c) Calculated number of the NKB and NK3R neurons in the ARC and MPO in all four groups of rats ( per group). All observations from individual animal were averaged for that animal, and then these single numbers of each animal were used to calculate the group mean. The data is expressed as the mean with SEM. N and MS groups were not shown in the figure. N: normal, M: model, MS: saline, and CHM: Chinese herb mixture. versus N; versus M; versus N.
