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Evidence-Based Complementary and Alternative Medicine
Volume 2014, Article ID 246748, 10 pages
Research Article

Coenzyme Q0 from Antrodia cinnamomea in Submerged Cultures Induces Reactive Oxygen Species-Mediated Apoptosis in A549 Human Lung Cancer Cells

1Department of Biochemical Science and Technology, National Taiwan University, No. 1, Roosevelt Road Section 4, Taipei 10617, Taiwan
2The Development Center for Biotechnology, No. 101, Lane 169, Kangning Street, New Taipei City 22180, Taiwan

Received 10 June 2014; Revised 12 September 2014; Accepted 16 September 2014; Published 6 November 2014

Academic Editor: Yew-Min Tzeng

Copyright © 2014 Cheng-Han Chung et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


We investigated the anticancer effects of Antrodia cinnamomea, a medicinal mushroom from Taiwan, on A549 human lung cancer cells using the ethyl acetate extract from submerged culture filtrates. Our results showed that 2,3-dimethoxy-5-methyl-1,4-benzoquinone (coenzyme Q0; CoQ0) derived from A. cinnamomea submerged culture filtrates has anticancer activity. CoQ0 treatment reduced the viability of A549, HepG2, and SW480 cancer cell lines. Furthermore, CoQ0 induced reactive oxygen species (ROS) generation and apoptosis in A549 cells, which was inhibited by the antioxidant ascorbic acid. To our knowledge, these data demonstrate for the first time that CoQ0 derived from A. cinnamomea submerged culture filtrates exerts its anticancer effect through the induction of ROS-mediated apoptosis in A549 human lung cancer cells.