<i>Persea declinata</i> (Bl.) Kosterm Bark Crude Extract Induces Apoptosis in MCF-7 Cells via  <b>G<sub>0</sub>/G<sub>1</sub></b> Cell Cycle Arrest, Bcl-2/Bax/Bcl-xl Signaling Pathways, and ROS Generation

<table>(a) Effect of PDM on cell cycle arrest. The MCF-7 cells were treated with DMSO, doxorubicin, or PDM for 24 h, stained with BrdU and Phospho-histone H3, and subjected to the Cellomics ArrayScan HCS reader for cell cycle analysis. (b) Representative bar charts indicating that PDM treatment caused no significant changes in BrdU or Phospho-histone H3 fluorescence intensities, and consequently induced no cell cycle arrest in the treated MCF-7 cells. Data were expressed as the mean ± SD of fluorescence intensity readings for three independent experiments. (c) Cells were stained with Hoechst and the distribution in G<sub>0</sub>/G<sub>1</sub>, S, and G2/M cell cycle phases was determined with Cellomics High Content Screening.</table>

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Figure 3: <i>Persea declinata</i> (Bl.) Kosterm Bark Crude Extract Induces Apoptosis in MCF-7 Cells via  <b>G<sub>0</sub>/G<sub>1</sub></b> Cell Cycle Arrest, Bcl-2/Bax/Bcl-xl Signaling Pathways, and ROS Generation 

Figure 3 | Persea declinata (Bl.) Kosterm Bark Crude Extract Induces Apoptosis in MCF-7 Cells via  G0/G1 Cell Cycle Arrest, Bcl-2/Bax/Bcl-xl Signaling Pathways, and ROS Generation 