ERK1/2-Egr-1 Signaling Pathway-Mediated Protective Effects of Electroacupuncture in a Mouse Model of Myocardial Ischemia-Reperfusion

Figure 6

Combination of EA with ERK1/2 inhibitor did not produce more protection against myocardial I/R injury. Combination of EA and U0126 treatment was conducted to investigate the additive effects on ERK1/2/Egr-1 downregulation or the cardiac protective role. Western blot bands and corresponding densitometric analysis of Egr-1 and p-ERK1/2 are shown in (a–c) (𝑛=3/group). The myocardial levels of TNF-α and IL-1β ((d-e), 𝑛=3/group) as well as the serum level of cTnI (f, 𝑛=6/group) were determined.

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