Inhibitory and Acceleratory Effects of Inonotus obliquus on Tyrosinase Activity and Melanin Formation in B16 Melanoma Cells
Table 2
Effects of test compounds and kojic acid on cell viability and apoptosis rate in B16 melanoma cells. Control groups (from wells without test material or kojic acid) were set as 100% for cell viability and set as 0% for apoptosis rate. Experimental groups were expressed as a percentage of controls (mean ± SD).
Compounds
Cell viability
Apoptosis rate
100 μM
200 μM
400 μM
100 μM
200 μM
400 μM
Control
100
100
100
0
0
0
Fa-a
88.31 ± 3.21*
75.13 ± 2.65*
65.32 ± 4.41
9.01 ± 2.21
12.21 ± 3.03
15.31 ± 2.90
Fa-b
96.45 ± 3.08*
85.32 ± 4.13*
70.02 ± 2.05
5.11 ± 4.01
6.51 ± 3.31
10.09 ± 4.31
Fb-a
99.34 ± 1.53*
99.43 ± 1.41
98.24 ± 1.35
3.91 ± 3.27
5.10 ± 2.61*
6.07 ± 3.53**
Fb-b
99.98 ± 2.56**
96.26 ± 2.61
94.56 ± 3.03
0.31 ± 4.37***
2.62 ± 3.33***
5.12 ± 2.35*
Fc-a
73.53 ± 3.11**
45.31 ± 2.56
40.51 ± 2.12
10.12 ± 1.14
25.44 ± 1.53***
30.51 ± 1.11***
Fd-a
96.70 ± 1.01
97.12 ± 1.12
85.03 ± 1.51
1.21 ± 2.31
2.15 ± 3.92
7.34 ± 3.28
Kojic acid
92.43 ± 4.41***
86.08 ± 2.12**
70.91 ± 3.12**
9.45 ± 1.15
12.36 ± 1.19
15.05 ± 2.21
< 0.001, < 0.01, and < 0.05 compared with the control.
