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Evidence-Based Complementary and Alternative Medicine
Volume 2014, Article ID 274786, 10 pages
http://dx.doi.org/10.1155/2014/274786
Research Article

Effect of Uncaria tomentosa Extract on Apoptosis Triggered by Oxaliplatin Exposure on HT29 Cells

1University Hospital of Santa Maria, Federal University of Santa Maria (UFSM), Avenida Roraima, Prédio 20, 97105-900 Santa Maria, RS, Brazil
2Postgraduate Program in Pharmaceutical Sciences, UFSM, 97105-900 Santa Maria, RS, Brazil
3Laboratory of Biotechnology and Animal Reproduction, UFSM, 97105-900 Santa Maria, RS, Brazil
4Postgraduate Program in Biochemistry and Toxicology, UFSM, 97105-900 Santa Maria, RS, Brazil
5Postgraduate Program in Agronomy, UFSM, 97105-900 Santa Maria, RS, Brazil
6Lutheran University of Brazil (ULBRA), BR 287, Km 252, 97020-001 Santa Maria, RS, Brazil
7Herbarium Laboratório Botânico Ltda., Avenida Santos Dumont 1100, 83403-500 Colombo, PR, Brazil

Received 17 June 2014; Accepted 17 October 2014; Published 11 November 2014

Academic Editor: Vincenzo De Feo

Copyright © 2014 Liliane Z. de Oliveira et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background/Aim. The use of herbal products as a supplement to minimize the effects of chemotherapy for cancer treatment requires further attention with respect to the activity and toxicity of chemotherapy. Uncaria tomentosa extract, which contains oxindole alkaloids, is one of these herbal products. The objective of this study was to evaluate whether Uncaria tomentosa extract modulates apoptosis induced by chemotherapy exposure. Materials and Methods. Colorectal adenocarcinoma cells (HT29 cells) were grown in the presence of oxaliplatin and/or Uncaria tomentosa extract. Results. The hydroalcoholic extract of Uncaria tomentosa enhanced chemotherapy-induced apoptosis, with an increase in the percentage of Annexin positive cells, an increase in caspase activities, and an increase of DNA fragments in culture of the neoplastic cells. Moreover, antioxidant activity may be related to apoptosis. Conclusion. Uncaria tomentosa extract has a role for cancer patients as a complementary therapy. Further studies evaluating these beneficial effects with other chemotherapy drugs are recommended.