Administration Dependent Antioxidant Effect of Carica papaya Seeds Water Extract
Figure 1
Differential centrifugation of crude C. papaya seeds water extract. The crude extract was subjected to a short run at 800 g by Centrifuge 4236 A (Beckman Coulter Inc., Brea, CA, USA) to obtain a first pellet and a supernatant that was further centrifuged at 16000 g to obtain a second pellet and supernatant. The second supernatant was centrifuged at 100000 g to obtain a third pellet and supernatant. This last supernatant was centrifuged at 4750 g by using a Centricon centrifugal filter device with an Ultracel YM-10 regenerated cellulose membrane that permits the ultrafiltration into the lower chamber of substances with molecular weight kDa, while substances with kDa are collected in the upper chamber (Merck Millipore, Billerica, MA, USA).
