Administration Dependent Antioxidant Effect of Carica papaya Seeds Water Extract
Viability and morphology of Detroit 550 fibroblasts treated with C. papaya seeds water extract. (a) Viability was assessed by MTT assay. Detroit 550 fibroblasts were treated with 1 or 2 mg/mL C. papaya (Cp) seeds water extract for 1, 2, 4, 8, and 24 hours (h). The values are reported as percentage of the control untreated fibroblasts considered as 100%. Each value represents the mean ± SE of six independent experiments, each done in duplicate. Single star indicates value significantly different from untreated control fibroblasts (). Two stars indicate value significantly different from the one star value (). (b) LM (A, D), SEM (B, E), and TEM (C, F) micrographs of Detroit 550 fibroblasts untreated (A–C) or treated with 1 mg/mL of C. papaya seeds water extract for 4 h (D–F). (A, D): H-E stained fibroblasts; bars = 20 μm. (B, E): bars = 5 μm. (C, F): the inset is a magnification showing microvilli; bars = 5 μm. (c) Percentages of normal, apoptotic, necrotic, and mitotic Detroit 550 fibroblasts after treatment with 1 or 2 mg/mL C. papaya (Cp) seeds water extract for 1, 2, 4, and 8 h. At least 500 fibroblasts for each treatment were scored on H-E stained slides.
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