Evidence-Based Complementary and Alternative Medicine / 2014 / Article / Fig 2

Research Article

Administration Dependent Antioxidant Effect of Carica papaya Seeds Water Extract

Figure 2

Viability and morphology of Detroit 550 fibroblasts treated with C. papaya seeds water extract. (a) Viability was assessed by MTT assay. Detroit 550 fibroblasts were treated with 1 or 2 mg/mL C. papaya (Cp) seeds water extract for 1, 2, 4, 8, and 24 hours (h). The values are reported as percentage of the control untreated fibroblasts considered as 100%. Each value represents the mean ± SE of six independent experiments, each done in duplicate. Single star indicates value significantly different from untreated control fibroblasts ( ). Two stars indicate value significantly different from the one star value ( ). (b) LM (A, D), SEM (B, E), and TEM (C, F) micrographs of Detroit 550 fibroblasts untreated (A–C) or treated with 1 mg/mL of C. papaya seeds water extract for 4 h (D–F). (A, D): H-E stained fibroblasts; bars = 20 μm. (B, E): bars = 5 μm. (C, F): the inset is a magnification showing microvilli; bars = 5 μm. (c) Percentages of normal, apoptotic, necrotic, and mitotic Detroit 550 fibroblasts after treatment with 1 or 2 mg/mL C. papaya (Cp) seeds water extract for 1, 2, 4, and 8 h. At least 500 fibroblasts for each treatment were scored on H-E stained slides.

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