Administration Dependent Antioxidant Effect of Carica papaya Seeds Water Extract
Figure 4
Antiapoptotic activity of C. papaya seeds water extract on Detroit 550 fibroblasts. Detroit 550 fibroblasts were treated with 10−2 M cycloheximide (CHX, in culture medium) or 10 μg/mL puromycin (PMC, in culture medium) either alone or in combination with 1 or 2 mg/mL of C. papaya (Cp) extract for 24 or 6 hours (h), respectively. (a) Viability was assessed by MTT assay. Stars show significant value with the corresponding 24 or 6 h values (). (b) Percentages of normal and apoptotic fibroblasts. At least 500 fibroblasts for each treatment were scored on H-E stained slides. Single star indicates values that differ significantly from the corresponding normal or apoptotic ones (). Each value represents the mean ± SE of six independent experiments, each done in duplicate.
