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Evidence-Based Complementary and Alternative Medicine
Volume 2014, Article ID 287063, 5 pages
Research Article

Observation of the Primo Vessel Approaching the Axillary Lymph Node with the Fluorescent Dye, DiI

1Nano Primo Research Center, Advanced Institute of Convergence Technology, Seoul National University, Suwon 443-270, Republic of Korea
2Physics Department, Gachon University, Seongam 461-701, Republic of Korea
3Department of Cosmetology, Hanseo University, Seosan 356-706, Republic of Korea
4Department of Acupuncture & Moxibustion, Gwangju Medical Hospital, Wonkwang University, Gwangju 503-310, Republic of Korea

Received 25 July 2014; Revised 17 September 2014; Accepted 26 September 2014; Published 16 November 2014

Academic Editor: Kyung A. Kang

Copyright © 2014 Su Youn Park et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The primo vascular system (PVS) floating in lymph fluid has mostly been observed in large caliber ducts around the caudal vena cava and the thoracic duct of rabbits, rats, and mice. But the PVS has not been traced up to the lymph nodes. It has not been established whether the PVS leaves the lymph vessel through the lymph vessel wall or it enters the lymph nodes. Therefore, observing the PVS entering a lymph node, for example, the axillary node, is desirable. In the current work, we traced the PVS approaching up to the surface of axillary node of a rat. The method used for this study was based upon a method that was recently developed to detect the PVS in the lymph duct from the inguinal to the axillary nodes in the skin of a rat by injecting Alcian blue into the inguinal node. However, the Alcian blue blurred near the lymph nodes and tracing the PVS up to the lymph nodes has not been possible. The current method clearly showed the PVS approaching the axillary node.