Differential Effects of Naja naja atra Venom on Immune Activity
Figure 1
NNAV increased innate and humoral immune activity. NNAV (20~80 μg/kg) was administered orally to ICR mice for 21 days, and control mice were given water instead. (a) The effector cells isolated from the spleens of NNAV-treated or control mice were cultured with target cells (YAC-1 cell line) for 4 h at the ratio of 10 : 1. The NK cell activity was determined using the LDH release assay. Values represent mean values ± SD from 4 mice per group; triplicates were used for each mouse. (versus control). (b) Mice in model and NNAV-treated group were immunized with 0.2 mL 5% SRBC on day 16 after treatment, and serum was collected on day 21. Mouse anti-SRBC antibody (Ab) levels were measured by haemolysis test. Values represent mean values ± SD for 10 mice per group. (versus model). (c) Isolated splenocytes from NNAV-treated or control mice were stimulated with LPS (10 μg/mL) for 48 h and the proliferation rate of B cells was determined using CCK-8. Data represent mean values ± SD for 4 mice per group; triplicates were used for each mouse. (versus control).
