Ganoderma lucidum Polysaccharides Reduce Lipopolysaccharide-Induced Interleukin-1β Expression in Cultured Smooth Muscle Cells and in Thoracic Aortas in Mice
Figure 1
EORP reduces IL-1β protein expression in LPS-treated HASMCs. (a) and (b) HASMCs were treated with 10 μg/mL of LPS for the indicated time (a) or with the indicated concentration of LPS for 24 h (b). (c) HASMCs were incubated for 24 h with 10 μg/mL of EORP and then for 24 h with 10 μg/mL of LPS in the continued presence of the same concentration of EORP, and then IL-1β expression was measured in cell lysates by Western blotting. α-Tubulin was used as the loading control. (d) The cells were treated as in panel (c), and then the distribution of IL-1β was analyzed by immunofluorescent microscopy. IL-1β expression is indicated by green fluorescence (FITC) and nuclei by blue fluorescence (DAPI). Bar = 100 μm. In panels (a), (b), and (c), the data are expressed as a fold value compared to the control value and are the means ± SEMs (). * as compared to the untreated (control) cells. † as compared to the LPS-treated cells.
