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Evidence-Based Complementary and Alternative Medicine
Volume 2014 (2014), Article ID 309095, 11 pages
Research Article

Intracellular ROS Scavenging Activity and Downregulation of Inflammatory Mediators in RAW264.7 Macrophage by Fresh Leaf Extracts of Pseuderanthemum palatiferum

1School of Biology, Institute of Science, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand
2School of Pharmacology, Institute of Science, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand

Received 18 December 2013; Accepted 5 February 2014; Published 16 March 2014

Academic Editor: Yoshiji Ohta

Copyright © 2014 Patcharawan Sittisart and Benjamart Chitsomboon. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Beneficial antioxidant phytochemicals are found in many medicinal plants. Pseuderanthemum palatiferum (PP), a well-known Vietnamese traditional medicinal plant in Thailand, has long been used in folk medicine for curing inflammatory diseases, often with limited support of scientific research. Therefore, this study aimed to determine antioxidant and modulation of inflammatory mediators of ethanol and water extracts of PP (EEP and WEP, resp.). WEP had significantly higher phenolic and flavonoid levels and DPPH radical scavenging activity than EEP. However, EEP exhibited greater reducing power than WEP. A greater decrease of tert-butyl hydroperoxide-induced oxidative stress in RAW264.7 macrophage cells was also observed with EEP. Modulation of inflammatory mediators of EEP and WEP was evaluated on LPS plus IFN-γ-stimulated RAW264.7 cells. EEP more potently suppressed LPS plus IFN-γ-induced nitric oxide (NO) production than WEP. Both EEP and WEP also suppressed the expression of iNOS and COX-2 protein levels. Collectively, these results suggest that PP possesses strong antioxidant and anti-inflammatory properties.