Intracellular ROS Scavenging Activity and Downregulation of Inflammatory Mediators in RAW264.7 Macrophage by Fresh Leaf Extracts of Pseuderanthemum palatiferum
Cellular radical scavenging activity in tBuOOH-activated RAW264.7 cells. Intracellular ROS level generated in cells was measured by the DCFH-DA. RAW264.7 cells were pretreated with indicated concentrations of antioxidants (a), EEP (b) or WEP (c), for 24 h prior to take-up of 20 M DCFH-DA for 30 min. Results are mean ± SEM and are representative of three independent experiments with similar results. Points marked with different letters are significantly different at when compared at the same time point as determined by two-way ANOVA.