Table 6: IC50 values ( g/mL) of P. bleo leaf extracts and fractions on different cell lines.

Cell lineExtracts and fractions (IC50: g/mL)Positive control (IC50: g/mL)Negative controlReferences
MethanolWaterHexane Dichloromethane Ethylacetate

4T1 >50>50NANANACisplatin (NA)NA[32]
CasKi 40.589.5NA58Doxorubicin (6 × 10−3)NA[36]
CEM-ssNANAVC[8]
HT29 and HCT116>30NA>30>30>30NAVC[8]
41.667.5NA22Doxorubicin (3.6 × 10−1)NA[36]
KB6.528NA4.5Doxorubicin (1.2 × 10−2)NA[36]
MCF-7>30NA>30>30>30NAVC[8]
3925NA28Doxorubicin (7.5 × 10−2)NA[36]
MRC-5NADoxorubicin (5.5 × 10−1)NA[36]
NIH/3T3≥200≥200NANANACisplatinNA[32]
Saos-2NANANANACisplatinNA[39]
T-47D2NANANANADNase I VC[40]
V79NANANANANitracrineVC[22]

IC50: 50% of maximum cell inhibition. IC50 < 20  g/mL is considered active, 100 > IC50 > 20  g/mL is relatively active, and IC50 > 100 is not active [8].
(—): no activity.
4T1: mouse mammary cancer cell line; CasKi: human cervical carcinoma cell line; CEM-ss: human T-4 lymphoblastoid cell line; HT29 and HCT116: human colon carcinoma cell line; KB: human nasopharyngeal epidermoid carcinoma cell line; MCF-7: hormone dependent breast carcinoma cell line; MRC-5: normal human fibroblast cell lines; NIH/3T3: normal mouse fibroblast cell line; Saos-2: human osteosarcoma cell line; T-47D: human breast carcinoma cell line; V79: Chinese hamster lung fibroblasts.
NA: not available.
VC: vehicle control.