Immunosuppressive Effects of A-Type Procyanidin Oligomers from Cinnamomum tamala

Figure 3

Cytotoxicity on splenocytes and inhibition on ConA-induced splenocyte proliferation of CT-F. (a) Cytotoxicity of CT-F on BALB/c mice splenocytes. The cells were incubated with different concentration of CT-F for 48 h. And the cell viability was measured by MTT assay. (b) Inhibition of CT-F on ConA-induced splenocyte proliferation. BALB/c mice splenocytes (4 × 10⁵ cells/well) were stimulated by ConA (2 μg/mL) for 48 h in the presence of CT-F. Cells were then pulsed with 0.25 μCi [³H]thymidine 8 h before the end of the experiment and were assessed for [³H]thymidine incorporation. Effect of CSA (1 μM) was set as positive group. Results are mean ± S.D. , treatment group versus control.

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