Protection of Tong-Sai-Mai Decoction against Apoptosis Induced by H₂O₂ in PC12 Cells: Mechanisms via Bcl-2-Mitochondria-ROS-INOS Pathway

Figure 13

INOS-specific inhibitor blocked H₂O₂-induced apoptosis in PC12 cells. The cells were exposed to different treatments for 24 h, respectively. The percentage of PC12 cells apoptosis was assessed by the FCM assay. The cells were treated with 200 μmol/L H₂O₂, 0.1 mmol/L AG, INOS-specific inhibitor, 0.1 mmol/L AG, INOS-specific inhibitor with 200 μmol/L H₂O₂, and 0.05, 0.5, and 5 mg/mL TSM with 200 μmol/L H₂O₂, indicating that AG can partly block the H₂O₂-induced PC12 cells apoptosis. and as compared with the control group; and as compared with the H₂O₂ group.