Protection of Tong-Sai-Mai Decoction against Apoptosis Induced by H₂O₂ in PC12 Cells: Mechanisms via Bcl-2-Mitochondria-ROS-INOS Pathway

Figure 5

Nuclear staining to evaluate induction of PC12 cells apoptosis by H₂O₂ and the cytoprotection of TSM. The nuclear condensation was assessed by using Hoechst 33258 for PC12 cells. (a) Control group; (b) PC12 cells exposed to 200 μmol/L H₂O₂ clearly showed the apoptotic nuclear condensation; ((c), (d), and (e)) PC12 cells, cotreated with 0.05, 0.5, and 5 mg/mL TSM with 200 μmol/L H₂O₂, did not obviously exhibit such nuclear condensation.

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