Protection of Tong-Sai-Mai Decoction against Apoptosis Induced by H₂O₂ in PC12 Cells: Mechanisms via Bcl-2-Mitochondria-ROS-INOS Pathway

Figure 6

TSM protected PC12 cells against apoptosis induced by H₂O₂. (a) Control group. The cells were treated with 200 μmol/L H₂O₂ (b) and 0.05, 0.5, and 5 mg/mL TSM in the presence of 200 μmol/L H₂O₂ ((c), (d), and (e)). Annexin V+/PI− population (LR in a diagram) indicated early apoptosis and annexin V+/PI+ population (UR in diagram) indicated late apoptosis. The analysis was performed in triplicate and representative data was shown. The percentage of PC12 cells apoptosis was assessed by annexin V/PI FCM assay. and as compared with the control group; and as compared with the H₂O₂ group.

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