Generation of the Bovine Viral Diarrhea Virus E0 Protein in Transgenic Astragalus and Its Immunogenicity in Sika Deer
Figure 2
Protein analysis in transgenic Astragalus extract. (a) ELISA test to determine the presence of the antigen E0 in transgenic Astragalus leaves extract. ELISA protocol was described in methods section. Transgenic Astragalus group (the recombinant proteins extracted from transgenic Astragalus leaves); positive control (E0 protein expressed in BHK-21 cells); negative control (proteins extracted from wild-type Astragalus). (b) Coomassie brilliant blue staining of protein extracted from transgenic Astragalus leaves analyzed by 12% of SDS-PAGE. M: protein markers, 1: negative control, 2–4: transgenic Astragalus groups, 5: positive control. (c) Western blot analysis showing immune activity of the E0 protein expressing in transgenic Astragalus. In transgenic Astragalus group (lanes 1, 4) and positive control (lane 2 E0 protein expressed in BHK-21 cells) a specific band of 50 kDa was detected. Lane 3: negative control (proteins extracted from wild-type Astragalus).
