Optimization of Cat’s Whiskers Tea (Orthosiphon stamineus) Using Supercritical Carbon Dioxide and Selective Chemotherapeutic Potential against Prostate Cancer Cells

Figure 8

Chemical characterization of the extract B2 on mass spectrum of GC-MS was conducted with the help of the Metabolites Spectral Database and NIST (National Institute of Standards and Technology) Library (Ezhilan and Neelamegam, 2012). The identification of the major chemical components was based on similarity index (SI), Wiley 8 computer library. The mass spectrum of the unknown component was compared with the spectrum of the known components stored in the library. The retention time, nomenclature, molecular weight, structure, and composition of the major components were recorded. The pie charts depict the relative chemical compositions of subfractions. The chart illustrates that the dominant peaks of B2 are acetic acid (3-hydroxy-7-isopropenyl-1,4a-dimethyl-octahydronaphthalen-2-yl) ester (27.01%), dimethyl-4-(1-methylethylidene)-2,4,6,7,8,8a-hexahydro-5(1H)-azulenone (26.86%), and anthracene, 9-(2-propenyl) (25.33%). The details of the peaks are given in Table 1.