Auraptene in the Peels of Citrus kawachiensis (Kawachi Bankan) Ameliorates Lipopolysaccharide-Induced Inflammation in the Mouse Brain

Figure 5

Effect of p.o. administration of the dried peels of C. kawachiensis on LPS-induced expression of GFAP and COX-2 in the mouse hippocampus. (a) Sagittal sections were stained with antibodies specific for GFAP (red: I, IV, VII, and X) or COX-2 (green: II, V, VIII, and XI). Merged pictures (III, VI, IX, XII) show cells that co-expressed GFAP and COX-2 (yellow cells). Scale bar indicates 100 μm. (b) GFAP-positive cells and (c) COX-2-positive cells were counted by use of ImageJ software. Values are means ± SEM (𝑛 = 8~10 for each group). Symbols indicate significant differences versus nontreated group (^∗∗𝑃<0.01 and ^∗∗∗𝑃<0.001) and versus LPS-treated group (^#𝑃<0.05, ^##𝑃<0.01, and ^###𝑃<0.001).

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