Ethanol Extract of Alismatis rhizome Inhibits Adipocyte Differentiation of OP9 Cells
Figure 1
Effects of AOE on viability and lipid accumulation in OP9 cells. (a) Confluent OP9 cells were differentiated into adipocytes in medium containing adipogenic inducers for varying time intervals in the presence or absence of AOE (20 and 40 μg/mL), as described in Materials and Methods. Effects of AOE on cell viability were measured by MTT assay and data were represented as relative cell viability values. ((b) and (c)) Cells were treated with adipogenic inducers to trigger differentiation into adipocytes in the presence or absence of various concentrations of AOE. After 5 days of differentiation, the cells were subjected to Oil Red O staining for a qualitative (b) and quantitative (c) comparison of intracellular lipid accumulation. The bars represent fold increases compared with ND groups. Data are presented as mean SD values of at least 3 independent experiments. ; compared to the D5 group. ND: no differentiation, D5: differentiation day 5, and N: negative control (20 μg/mL Radix Astragali extract).
