Evidence-Based Complementary and Alternative Medicine / 2014 / Article / Fig 1

Research Article

Moxibustion Activates Macrophage Autophagy and Protects Experimental Mice against Bacterial Infection

Figure 1

Autophagy is accelerated by moxibustion. Macrophages were collected from mice with or without moxibustion as indicated, fixed, and stained with anti-LC3 antibody, and LC3 staining was visualized by confocal fluorescence microscopy. Representative images (a) and the means of LC3 puncta/cell counted from 20 individual cells (b) are shown. . Macrophages were lysed and equal mixtures of whole cell extracts were detected by western blots with anti-LC3 and anti-actin antibodies (c).