In Vivo and In Vitro Antitumor Effects of Platycodin D, a Saponin Purified from Platycodi Radix on the H520 Lung Cancer Cell

Table 1

Primary antisera and detection kits used in this study.

Antisera or detection kits	Code	Source	Dilution
Primary antisera
Anticleaved caspase-3 (Asp175) polyclonal antibody	9661	Cell Signaling Technology Inc, MA, USA	1 : 400
Anticleaved PARP (Asp214) rat specific antibody	9545	Cell Signaling Technology Inc, MA, USA	1 : 100
Antitumor necrosis factor-α (4E1) antibody	sc-130349	Santa Cruz Biotechnology, CA, USA	1 : 200
Anticyclooxygenase (murine) polyclonal antibody	160126	Cayman Chemical., MI, USA	1 : 200
Anti-nitric oxide synthase2 (N-20) polyclonal antibody	sc-651	Santa Cruz Biotechnology, CA, USA	1 : 100
Detection kits
Vectastain Elite ABC Kit	PK-6200	Vector Lab. Inc., CA, USA	1 : 50
Peroxidae substrate kit	SK-4100	Vector Lab. Inc., CA, USA	1 : 50

All antisera were diluted by phosphate buffered saline (pH 7.2).