Intestinal Transportations of Main Chemical Compositions of Polygoni Multiflori Radix in Caco-2 Cell Model

Table 1

Elution procedure and detection wavelength of high performance liquid chromatography assays.

(a) Isocratic elution procedure, detection wavelength, and 𝑅_𝑡 of EN, AE, RL, PL, and EG

Sample	Detection wavelength (nm)	B%	𝑅𝑡 (min)
EN	287	75	11.24
AE	254	80	5.042
RL	306	45	8.423
PL	306	40	6.568
EG	280	55	12.11

(b) Gradient elution procedure, detection wavelength, and 𝑅_𝑡 of TSG and RN

Sample	Detection; wavelength (nm)	B%			𝑅𝑡 (min)
		0 min	5 min	10 min
TSG	320	40	55	70	6.513
RN	254	85	/	90	5.307

Mobile phase A: 0.1% H₃PO₄.
Mobile phase B: methanol.