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Evidence-Based Complementary and Alternative Medicine
Volume 2014, Article ID 484269, 9 pages
http://dx.doi.org/10.1155/2014/484269
Research Article

Paeonol Inhibits Proliferation of Vascular Smooth Muscle Cells Stimulated by High Glucose via Ras-Raf-ERK1/2 Signaling Pathway in Coculture Model

1Key Laboratory of Xin’an Medicine, Ministry of Education, Anhui Province Key Laboratory of R&D of Chinese Medicine, Anhui University of Traditional Chinese Medicine, Hefei 230038, China
2School of Pharmacy, Anhui University of Traditional Chinese Medicine, Shihe Road 45, Hefei, Anhui 230031, China

Received 19 February 2014; Revised 29 April 2014; Accepted 18 May 2014; Published 5 June 2014

Academic Editor: Hao Xu

Copyright © 2014 Junjun Chen et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. A. Didangelos, D. Simper, C. Monaco, and M. Mayr, “Proteomics of acute coronary syndromes,” Current Atherosclerosis Reports, vol. 11, no. 3, pp. 188–195, 2009. View at Publisher · View at Google Scholar · View at Scopus
  2. J. C. Wang and M. Bennett, “Aging and atherosclerosis: mechanisms, functional consequences, and potential therapeutics for cellular senescence,” Circulation Research, vol. 111, no. 2, pp. 245–259, 2012. View at Publisher · View at Google Scholar · View at Scopus
  3. J. A. Beckman, M. A. Creager, and P. Libby, “Diabetes and atherosclerosis epidemiology, pathophysiology, and management,” Journal of the American Medical Association, vol. 287, no. 19, pp. 2570–2581, 2002. View at Google Scholar · View at Scopus
  4. L. Piconi, L. Quagliaro, R. Assaloni et al., “Constant and intermittent high glucose enhances endothelial cell apoptosis through mitochondrial superoxide overproduction,” Diabetes/Metabolism Research and Reviews, vol. 22, no. 3, pp. 198–203, 2006. View at Publisher · View at Google Scholar · View at Scopus
  5. S. M. Baumgartner-Parzer, L. Wagner, M. Pettermann, J. Grillari, A. Gessl, and W. Waldhausl, “High-glucose-triggered apoptosis in cultured endothelial cells,” Diabetes, vol. 44, no. 11, pp. 1323–1327, 1995. View at Google Scholar · View at Scopus
  6. S. Stintzing, M. Ocker, A. Hartner, K. Amann, L. Barbera, and D. Neureiter, “Differentiation patterning of vascular smooth muscle cells (VSMC) in atherosclerosis,” Virchows Archiv, vol. 455, no. 2, pp. 171–185, 2009. View at Publisher · View at Google Scholar · View at Scopus
  7. A. R. Keramati, R. Singh, A. Lin et al., “Wild-type LRP6 inhibits, whereas atherosclerosis-linked LRP6R611C increases PDGF-dependent vascular smooth muscle cell proliferation,” Proceedings of the National Academy of Sciences of the United States of America, vol. 108, no. 5, pp. 1914–1918, 2011. View at Publisher · View at Google Scholar · View at Scopus
  8. K. Sternberg, M. Gratz, K. Koeck et al., “Magnesium used in bioabsorbable stents controls smooth muscle cell proliferation and stimulates endothelial cells in vitro,” Journal of Biomedical Materials Research B, Applied Biomaterials, vol. 100, no. 1, pp. 41–50, 2012. View at Publisher · View at Google Scholar · View at Scopus
  9. C. Qin and Z. Liu, “In atherogenesis, the apoptosis of endothelial cell itself could directly induce over-proliferation of smooth muscle cells,” Medical Hypotheses, vol. 68, no. 2, pp. 275–277, 2007. View at Publisher · View at Google Scholar · View at Scopus
  10. T. P. Garrington and G. L. Johnson, “Organization and regulation of mitogen-activated protein kinase signaling pathways,” Current Opinion in Cell Biology, vol. 11, no. 2, pp. 211–218, 1999. View at Publisher · View at Google Scholar · View at Scopus
  11. J. Huang, L.-S. Li, D.-L. Yang, Q.-H. Gong, J. Deng, and X.-N. Huang, “Inhibitory effect of ginsenoside Rg1 on vascular smooth muscle cell proliferation induced by PDGF-BB is involved in nitric oxide formation,” Evidence-Based Complementary and Alternative Medicine, vol. 2012, Article ID 314395, 12 pages, 2012. View at Publisher · View at Google Scholar · View at Scopus
  12. L. Labrecque, S. Lamy, A. Chapus et al., “Combined inhibition of PDGF and VEGF receptors by ellagic acid, a dietary-derived phenolic compound,” Carcinogenesis, vol. 26, no. 4, pp. 821–826, 2005. View at Publisher · View at Google Scholar · View at Scopus
  13. H. Shimizu, Y. Nakagawa, C. Murakami, N. Aoki, S. Kim-Mitsuyama, and H. Miyazaki, “Protein tyrosine phosphatase PTPεM negatively regulates PDGF β-receptor signaling induced by high glucose and PDGF in vascular smooth muscle cells,” The American Journal of Physiology—Cell Physiology, vol. 299, no. 5, pp. C1144–C1152, 2010. View at Publisher · View at Google Scholar · View at Scopus
  14. M. Kernt, A. S. Neubauer, R. G. Liegl et al., “Sorafenib prevents human retinal pigment epithelium cells from light-induced overexpression of VEGF, PDGF and PlGF,” The British Journal of Ophthalmology, vol. 94, no. 11, pp. 1533–1539, 2010. View at Publisher · View at Google Scholar · View at Scopus
  15. J. Ortmann, M. Veit, S. Zingg et al., “Estrogen receptor-α but not -β or GPER inhibits high glucose-induced human VSMC proliferation: potential role of ROS and ERK,” Journal of Clinical Endocrinology and Metabolism, vol. 96, no. 1, pp. 220–228, 2011. View at Publisher · View at Google Scholar · View at Scopus
  16. M. G. Sirois, M. Simons, and E. R. Edelman, “Antisense oligonucleotide inhibition of PDGFR-β receptor subunit expression directs suppression of intimal thickening,” Circulation, vol. 95, no. 3, pp. 669–676, 1997. View at Google Scholar · View at Scopus
  17. W. Lederle, N. Linde, J. Heusel et al., “Platelet-derived growth factor-B normalizes micromorphology and vessel function in vascular endothelial growth factor-A-induced squamous cell carcinomas,” The American Journal of Pathology, vol. 176, no. 2, pp. 981–994, 2010. View at Publisher · View at Google Scholar · View at Scopus
  18. S. M. Wilhelm, L. Adnane, P. Newell, A. Villanueva, J. M. Llovet, and M. Lynch, “Preclinical overview of sorafenib, a multikinase inhibitor that targets both Raf and VEGF and PDGF receptor tyrosine kinase signaling,” Molecular Cancer Therapeutics, vol. 7, no. 10, pp. 3129–3140, 2008. View at Publisher · View at Google Scholar · View at Scopus
  19. A. Das, U. Shergill, L. Thakur et al., “Ephrin B2/EphB4 pathway in hepatic stellate cells stimulates Erk-dependent VEGF production and sinusoidal endothelial cell recruitment,” The American Journal of Physiology—Gastrointestinal and Liver Physiology, vol. 298, no. 6, pp. G908–G915, 2010. View at Publisher · View at Google Scholar · View at Scopus
  20. S. Satofuka, A. Ichihara, N. Nagai et al., “(Pro)renin receptor-mediated signal transduction and tissue renin-angiotensin system contribute to diabetes-induced retinal inflammation,” Diabetes, vol. 58, no. 7, pp. 1625–1633, 2009. View at Publisher · View at Google Scholar · View at Scopus
  21. H. Li, M. Dai, and W. Jia, “Paeonol attenuates high-fat-diet-induced atherosclerosis in rabbits by anti-inflammatory activity,” Planta Medica, vol. 75, no. 1, pp. 7–11, 2009. View at Publisher · View at Google Scholar · View at Scopus
  22. J. Hu, Y.-L. Li, Z.-L. Li et al., “Chronic supplementation of paeonol combined with danshensu for the improvement of vascular reactivity in the cerebral basilar artery of diabetic rats,” International Journal of Molecular Sciences, vol. 13, no. 11, pp. 14565–14578, 2012. View at Publisher · View at Google Scholar · View at Scopus
  23. M. Dai, Q. Y. Liu, C. G. Gu, and H. Y. Zhang, “Inhibitory effect of paeonol on lipid peroxidational reaction and oxidational decorate of low density lipoprotein,” China Journal of Chinese Materia Medica, vol. 25, no. 10, pp. 625–627, 2000. View at Google Scholar · View at Scopus
  24. M. Dai, X. Zhi, D. Peng, and Q. Liu, “Inhibitory effect of paeonol on experimental atherosclerosis in quails,” China Journal of Chinese Materia Medica, vol. 24, no. 8, pp. 488–512, 1999. View at Google Scholar · View at Scopus
  25. Y.-Q. Wang, M. Dai, J.-C. Zhong, and D.-K. Yin, “Paeonol inhibits oxidized low density lipoprotein-induced monocyte adhesion to vascular endothelial cells by inhibiting the mitogen activated protein kinase pathway,” Biological and Pharmaceutical Bulletin, vol. 35, no. 5, pp. 767–772, 2012. View at Publisher · View at Google Scholar · View at Scopus
  26. L.-L. Pan, M. Dai, and W. Wang, “A new method for culturing endothelial cells from the rat aorta,” Chinese Pharmacological Bulletin, vol. 23, no. 3, pp. 410–413, 2007. View at Google Scholar · View at Scopus
  27. W. Wang, M. Dai, and L.-L. Pan, “A new method for culturing vascular smooth muscle cells from the rabbit aorta,” Chinese Pharmacological Bulletin, vol. 24, no. 11, pp. 1534–1537, 2008. View at Google Scholar · View at Scopus
  28. M. F. Fillinger, L. N. Sampson, J. L. Cronenwett, R. J. Powell, and R. J. Wagner, “Coculture of endothelial cells and smooth muscle cells in bilayer and conditioned media models,” Journal of Surgical Research, vol. 67, no. 2, pp. 169–178, 1997. View at Publisher · View at Google Scholar · View at Scopus
  29. N. S. Roy, C. Cleren, S. K. Singh, L. Yang, M. F. Beal, and S. A. Goldman, “Functional engraftment of human ES cell-derived dopaminergic neurons enriched by coculture with telomerase-immortalized midbrain astrocytes,” Nature Medicine, vol. 12, no. 11, pp. 1259–1268, 2006. View at Publisher · View at Google Scholar · View at Scopus
  30. H. Zhang, X. Jia, F. Han et al., “Dual-delivery of VEGF and PDGF by double-layered electrospun membranes for blood vessel regeneration,” Biomaterials, vol. 34, no. 9, pp. 2202–2212, 2013. View at Publisher · View at Google Scholar · View at Scopus
  31. J. A. Beckman, F. Paneni, F. Cosentino, and J. A. Beckman, “Diabetes and vascular disease: pathophysiology, clinical consequences, and medical therapy: part II,” European Heart Journal, vol. 108, no. 13, pp. 1527–1532, 2013. View at Google Scholar
  32. E. Stoyanova, M. Trudel, H. Felfly, W. Lemsaddek, D. Garcia, and G. Cloutier, “Vascular endothelial dysfunction in β-Thalassemia occurs despite increased eNOS expression and preserved vascular smooth muscle cell reactivity to NO,” PLoS ONE, vol. 7, no. 6, Article ID e38089, 2012. View at Publisher · View at Google Scholar · View at Scopus
  33. J. S. Petrofsky, “The effect of type-2-diabetes-related vascular endothelial dysfunction on skin physiology and activities of daily living,” Journal of Diabetes Science Technology, vol. 5, no. 3, pp. 657–667, 2011. View at Google Scholar · View at Scopus
  34. S. Sartini, S. Cosconati, L. Marinelli et al., “Benzofuroxane derivatives as multi-effective agents for the treatment of cardiovascular diabetic complications. Synthesis, functional evaluation, and molecular modeling studies,” Journal of Medicinal Chemistry, vol. 55, no. 23, pp. 10523–10531, 2012. View at Publisher · View at Google Scholar · View at Scopus
  35. J. Homsi and A. I. Daud, “Spectrum of activity and mechanism of action of VEGF/PDGF inhibitors,” Cancer Control, vol. 14, no. 3, pp. 285–294, 2007. View at Google Scholar · View at Scopus
  36. C. Ionescu, I. Berindan-Neagoe, C. Burz et al., “The clinical implications of platelet derived growth factor B, vascular endothelial growth factor and basic fibroblast growth factor in colorectal cancer,” Journal of Buon, vol. 16, no. 2, pp. 274–276, 2011. View at Google Scholar · View at Scopus
  37. N. Liu, J.-T. Liu, Y.-Y. Ji, and P.-P. Lu, “Effects and mechanisms of the functional parts of Dahuang Zhechong Pill containing serum on platelet-derived growth factor-stimulated proliferation of vascular smooth muscle cells,” Chinese Journal of Integrative Medicine, vol. 19, no. 6, pp. 432–438, 2013. View at Publisher · View at Google Scholar · View at Scopus
  38. D. Ge, N. Meng, L. Su et al., “Human vascular endothelial cells reduce sphingosylphosphorylcholine-induced smooth muscle cell contraction in co-culture system through integrin Β4 and Fyn,” Acta Pharmacologica Sinica, vol. 33, no. 1, pp. 57–65, 2012. View at Publisher · View at Google Scholar · View at Scopus
  39. C. S. Wallace, J. C. Champion, and G. A. Truskey, “Adhesion and function of human endothelial cells co-cultured on smooth muscle cells,” Annals of Biomedical Engineering, vol. 35, no. 3, pp. 375–386, 2007. View at Publisher · View at Google Scholar · View at Scopus
  40. T. Korff, S. Kimmina, G. Martiny-Baron, and H. G. Augustin, “Blood vessel maturation in a 3-dimensional spheroidal coculture model: direct contact with smooth muscle cells regulates endothelial cell quiescence and abrogates VEGF responsiveness,” The FASEB Journal, vol. 15, no. 2, pp. 447–457, 2001. View at Publisher · View at Google Scholar · View at Scopus
  41. L.-M. Zhao, L.-J. Ma, L.-X. Zhang, and J.-Z. Wu, “Shenmai injection inhibiting the extracellular signal regulated kinase-induced human airway smooth muscle proliferation in asthma,” Chinese Journal of Integrative Medicine, vol. 16, no. 4, pp. 331–336, 2010. View at Publisher · View at Google Scholar · View at Scopus
  42. Y. Mebratu and Y. Tesfaigzi, “How ERK1/2 activation controls cell proliferation and cell death is subcellular localization the answer?” Cell Cycle, vol. 8, no. 8, pp. 1168–1175, 2009. View at Google Scholar · View at Scopus