Capilliposide Isolated from Lysimachia capillipes Hemsl. Induces ROS Generation, Cell Cycle Arrest, and Apoptosis in Human Nonsmall Cell Lung Cancer Cell Lines
Figure 4
LC leads to ROS-mediated proliferative inhibition and apoptosis in H460 lung cancer cells. (a) ROS levels were determined 3 h later by flow cytometric analysis. The data demonstrated difference in the levels of intracellular ROS in control versus LC treated cells from a representative experiment. (b) The role of ROS in LC-induced apoptosis inhibition was assessed using ROS scavenger NAC. Cells were pretreatment with NAC (10 mM) for 1 h and then cotreated with 4 ug/mg LC for another 24 h. Impact of NAC on the apoptotic value was determined by annexin V-FITC/PI staining. (c) The role of ROS in LC-mediated proliferative inhibition was assessed using ROS scavenger NAC. The influence of NAC on LC-induced cytotoxicity was determined by MTT assay. All data are representative of at least three independent experiments. and indicate statistically significant differences versus control group.
