Research Article

Effect of Eugenol on Cell Surface Hydrophobicity, Adhesion, and Biofilm of Candida tropicalis and Candida dubliniensis Isolated from Oral Cavity of HIV-Infected Patients

Table 2

Antifungal concentrations of eugenol and fluconazole against planktonic and sessile cells of Candida dubliniensis and Candida tropicalis.

YeastEugenolFluconazole
MICaSMIC-1bSMIC-24cMICdSMICc

Candida dubliniensis
 ATCC MYA-6463753753758>512
 1317507501,5004>512
 2193757501,5008>512
 2483757507504>512
Candida tropicalis
 ATCC 28707375375375128>512
 233757507508>512
 1507507501,50032>512
 1763757501,50064>512

Minimum inhibitory concentration of the antifungal which resulted in total inhibition of visible planktonic cell growth; Minimum inhibitory concentration of the antifungal which resulted in total reduction in metabolic activity of sessile cells, using the XTT-reduction assay, after 1 h of adhesion; Minimum inhibitory concentration of the antifungal which resulted in total reduction in metabolic activity of sessile cells, using the XTT-reduction assay, after 24 h of biofilm formation; MIC was defined according to CLSI (2008) guidelines for fluconazole broth microdilution assays; The results are expressed as g/mL.