Evidence-Based Complementary and Alternative Medicine / 2014 / Article / Tab 2

Research Article

A Platform for Screening Potential Anticholinesterase Fractions and Components Obtained from Anemarrhena asphodeloides Bge for Treating Alzheimer’s Disease

Table 2

HPLC/Q-TOF MS analysis for accurate mass measurements of constituents in 60% ethanol fraction.

Number (min) Formula Selected ionTheoretical mass
Experimental mass
Error (ppm)Fragment ions in positive
(+) ion mode
Identification

129.8C45H76O20 M + Na +959.4822959.4833−1.1919, 757, 595, 433, 415, 271, 253Timosaponin N
231.7C45H74O19 M + Na +941.4717941.4726−1.0901, 739, 577, 415, 273, 255Timosaponin M
337.6C45H76O19 M + Na +943.4873943.4886−1.4903, 741, 579, 417, 273, 255Timosaponin BII
438.5C45H76O19 M + Na +943.4873943.4882−1.0903, 741, 579, 417, 273, 25525R-Timosaponin BII
539.2C45H76O19 M + Na +943.4873943.48710.2903, 741, 579, 417, 273, 25525S-Officinalisnin-I
654.9C45H74O18 M + Na +925.4767925.4788 −2.3741, 579, 417, 273, 255Timosaponin BIII
756.7C39H64O14 M + Na +779.4188779.4202−1.8739, 595, 433, 415, 273, 255Timosaponin G
857.6C39H66O14 M + Na +781.4345781.4367−2.8741, 579, 417, 399, 271, 253Anemarrhena saponin I
958.0C39H64O14 M + Na +779.4188779.4189−0.1739, 577, 415, 273, 255Isomer of timosaponin G
1058.6C39H64O14 M + Na +779.4188779.41840.5739, 595, 433, 415, 271, 25325(27)-ene-Anemarrhena saponin I
1160.4C39H64O14 M + Na +779.4188779.41870.1757, 595, 433, 415, 271, 253Timosaponin A2
1262.2C39H64O13 M + Na +763.4239763.42370.3579, 417, 273, 255Timosaponin AIV
1363.9C39H64O13 M + Na +763.4239763.4241−0.3579, 417, 273, 255Timosaponin AIII
1464.4C39H64O13 M + Na +763.4239763.42212.4579, 417, 273, 25525R-Timosaponin AIII
1575.8C33H54O8 M + Na +601.3711601.37031.3417, 273, 255Timosaponin AI