Evidence-Based Complementary and Alternative Medicine

Research Article

A Platform for Screening Potential Anticholinesterase Fractions and Components Obtained from Anemarrhena asphodeloides Bge for Treating Alzheimer’s Disease

Table 2

HPLC/Q-TOF MS analysis for accurate mass measurements of constituents in 60% ethanol fraction.


Number	(min)	Formula	Selected ion	Theoretical mass	Experimental mass	Error (ppm)	Fragment ions in positive (+) ion mode	Identification

1	29.8	C₄₅H₇₆O₂₀	M + Na⁺	959.4822	959.4833	−1.1	919, 757, 595, 433, 415, 271, 253	Timosaponin N
2	31.7	C₄₅H₇₄O₁₉	M + Na⁺	941.4717	941.4726	−1.0	901, 739, 577, 415, 273, 255	Timosaponin M
3	37.6	C₄₅H₇₆O₁₉	M + Na⁺	943.4873	943.4886	−1.4	903, 741, 579, 417, 273, 255	Timosaponin BII
4	38.5	C₄₅H₇₆O₁₉	M + Na⁺	943.4873	943.4882	−1.0	903, 741, 579, 417, 273, 255	25R-Timosaponin BII
5	39.2	C₄₅H₇₆O₁₉	M + Na⁺	943.4873	943.4871	0.2	903, 741, 579, 417, 273, 255	25S-Officinalisnin-I
6	54.9	C₄₅H₇₄O₁₈	M + Na⁺	925.4767	925.4788	−2.3	741, 579, 417, 273, 255	Timosaponin BIII
7	56.7	C₃₉H₆₄O₁₄	M + Na⁺	779.4188	779.4202	−1.8	739, 595, 433, 415, 273, 255	Timosaponin G
8	57.6	C₃₉H₆₆O₁₄	M + Na⁺	781.4345	781.4367	−2.8	741, 579, 417, 399, 271, 253	Anemarrhena saponin I
9	58.0	C₃₉H₆₄O₁₄	M + Na⁺	779.4188	779.4189	−0.1	739, 577, 415, 273, 255	Isomer of timosaponin G
10	58.6	C₃₉H₆₄O₁₄	M + Na⁺	779.4188	779.4184	0.5	739, 595, 433, 415, 271, 253	25(27)-ene-Anemarrhena saponin I
11	60.4	C₃₉H₆₄O₁₄	M + Na⁺	779.4188	779.4187	0.1	757, 595, 433, 415, 271, 253	Timosaponin A₂
12	62.2	C₃₉H₆₄O₁₃	M + Na⁺	763.4239	763.4237	0.3	579, 417, 273, 255	Timosaponin AIV
13	63.9	C₃₉H₆₄O₁₃	M + Na⁺	763.4239	763.4241	−0.3	579, 417, 273, 255	Timosaponin AIII
14	64.4	C₃₉H₆₄O₁₃	M + Na⁺	763.4239	763.4221	2.4	579, 417, 273, 255	25R-Timosaponin AIII
15	75.8	C₃₃H₅₄O₈	M + Na⁺	601.3711	601.3703	1.3	417, 273, 255	Timosaponin AI