NMR-Based Metabonomic Studies on Stomach Heat and Cold Syndromes and Intervention Effects of the Corresponding Formulas
Table 2
Identified differential metabolites in rat serum.
No.
Metabolite
SH versus control
SC versus control
SH versus SC
VIPa
FCb
VIPa
FCb
VIPa
FCb
1
Isoleucine
1.62
0.74
1.50
0.78
/
0.94
—
2
Leucine
1.36
0.83
1.57
0.82
/
1.01
—
3
Methionine
3.67
0.76
3.04
0.83
1.38
0.91
—
4
Lysine
1.28
0.63
1.78
0.83
/
0.76
—
5
Valine
/
0.84
—
1.54
0.75
/
1.12
—
6
Choline
3.14
0.68
/
0.94
—
2.31
0.72
7
Betaine
4.16
0.70
1.69
0.90
—
2.56
0.78
8
Creatine
2.16
0.80
/
0.93
—
1.30
0.86
9
Phosphocholine
2.99
0.70
2.43
0.79
1.01
0.89
—
10
Trimethylamine
1.18
0.51
1.36
0.68
1.21
0.74
11
-Glucose
/
1.03
—
2.12
0.54
1.56
1.91
12
-Glucose
1.13
1.29
—
1.19
0.76
1.54
1.70
13
Pyruvate
2.06
0.74
2.78
1.49
3.06
0.50
14
Lactate
2.67
0.79
5.01
1.45
4.90
0.54
15
Alanine
2.36
0.71
1.58
1.17
2.55
0.60
16
Glutamate
1.72
0.70
1.33
1.19
1.86
0.59
17
VLDL/LDL –CH2–
4.39
1.68
/
0.99
—
3.45
1.69
18
Lipid =CHCH2CH=
1.34
1.46
/
0.84
—
1.31
1.74
19
Lipid CH=CH
2.52
1.52
/
0.94
—
2.11
1.61
20
Acetoacetate
1.72
0.69
2.52
0.49
1.18
1.43
21
Acetone
1.09
1.15
—
1.68
0.76
1.91
1.50
VIP: variable importance in the projection; metabolites with VIP values ≥1.0 were considered significant; bFold change (FC) was calculated as the ratio of the mean metabolite levels between two groups; values were calculated from Student’s -test (equal variances assumed) or Mann-Whitney -test (equal variances not assumed) with a threshold of 0.05. The symbol “—’’ represents statistically nonsignificant values () and “/’’ denotes VIP values <1.0.
