Pleurotus eryngii Ameliorates Lipopolysaccharide-Induced Lung Inflammation in Mice
Figure 5
Effect of HTPE on histological changes in the lungs of LPS-induced ALI mice. Mice were administered, via the oral and intraperitoneal routes, HTPE (1 g/kg) and dexamethasone (DEX: 5 mg/kg, positive control group), respectively, 1 h before intranasal administration of LPS (10 μg/site). Vehicle (tap water, vehicle for HTPE) was also administered orally 1 h before intranasal administration of LPS (LPS group) or saline (vehicle for LPS, saline group (negative control group)). Lungs (-4) from each experimental group were processed for histological evaluation 6 h after LPS challenge. The result in each group was almost identical. Lung sections were stained with hematoxylin and eosin ((a), (b), (e), and (f)). Lung sections in (c) and (d) show the immunoreactivity of myeloperoxidase (a marker of neutrophils) and Mac-2 (a maker of macrophages). Scale bar: 100 μm. (a) Saline group (negative control group); ((b), (c), and (d))LPS group; (e) HTPE-treated LPS group; (f) DEX-treated LPS group (positive control group).
