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Evidence-Based Complementary and Alternative Medicine
Volume 2014, Article ID 542825, 12 pages
http://dx.doi.org/10.1155/2014/542825
Research Article

U-Bang-Haequi Tang: A Herbal Prescription that Prevents Acute Inflammation through Inhibition of NF-B-Mediated Inducible Nitric Oxide Synthase

1Department of Ophthalmology, Otolaryngology & Dermatology, Daegu Haany University, Daegu 706-828, Republic of Korea
2Medical Research Center for Globalization of Herbal Formulation, College of Oriental Medicine, Daegu Haany University, Daegu 706-828, Republic of Korea
3College of Pharmacy, Chosun University, Gwangju 501-759, Republic of Korea
4Sunlin University, Pohang 791-712, Republic of Korea
5College of Oriental Medicine, Dongguk University, Gyeongju 780-714, Republic of Korea
6Department of Pharmacology, Mudanjiang Medical University, Mudanjiang 157011, China

Received 18 February 2014; Revised 16 April 2014; Accepted 18 April 2014; Published 15 May 2014

Academic Editor: Bor-Show Tzang

Copyright © 2014 Min Hwangbo et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Since antiquity, medical herbs have been prescribed for both treatment and preventative purposes. Herbal formulas are used to reduce toxicity as well as increase efficacy in traditional Korean medicine. U-bang-haequi tang (UBT) is a herbal prescription containing Arctii fructus and Forsythia suspensa as its main components and has treated many human diseases in traditional Korean medicine. This research investigated the effects of UBT against an acute phase of inflammation. For this, we measured induction of nitric oxide (NO) and related proteins in macrophage cell line stimulated by lipopolysaccharide (LPS). Further, paw swelling was measured in carrageenan-treated rats. Carrageenan significantly induced activation of inflammatory cells and increases in paw volume, whereas oral administration of 0.3 or 1 g/kg/day of UBT inhibited the acute inflammatory response. In RAW264.7 cells, UBT inhibited mRNA and protein expression levels of iNOS. UBT treatment also blocked elevation of NO production, nuclear translocation of NF-B, phosphorylation of I-B induced by LPS. Moreover, UBT treatment significantly blocked the phosphorylation of p38 and c-Jun NH2-terminal kinases by LPS. In conclusion, UBT prevented both acute inflammation in rats as well as LPS-induced NO and iNOS gene expression through inhibition of NF-B in RAW264.7 cells.