U-Bang-Haequi Tang: A Herbal Prescription that Prevents Acute Inflammation through Inhibition of NF-B-Mediated Inducible Nitric Oxide Synthase

Figure 4

Inhibition of LPS-inducible NO and PGE₂ by UBT. (a) NO and (b) PGE₂ accumulations. Raw264.7 cells were treated with 0.2, 0.5, and 1.0 mg/mL of UBT for 1 h, and continuously with LPS (1 μg/mL) for the next 18 h. (c) TNF-α and IL-6 contents in culture medium. Raw264.7 cells were stimulated by LPS with or without 1.0 mg/mL UBT for 12 h. (d) TNF-α and IL-6 mRNA levels. THP-1 cells were stimulated by LPS with or without 1.0 mg/mL UBT for 12 h. Data represents the mean ± S.E.M. from four separate experiments (significant as compared with control, ^∗∗𝑃<0.01; significant as compared with LPS alone, ^#𝑃<0.05, ^##𝑃<0.01). UBT, U-bang-haequi tang.

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