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Evidence-Based Complementary and Alternative Medicine
Volume 2014, Article ID 543606, 11 pages
http://dx.doi.org/10.1155/2014/543606
Research Article

Myrcia bella Leaf Extract Presents Hypoglycemic Activity via PI3k/Akt Insulin Signaling Pathway

1Institute of Biosciences, São Paulo State University (UNESP), 18618-970 Botucatu, SP, Brazil
2Department of Biological Sciences, Faculty of Sciences, São Paulo State University (UNESP), 17033-360 Bauru, SP, Brazil
3Department of Physical Education, Faculty of Sciences, São Paulo State University (UNESP), Avenida Engenheiro Luiz Edmundo Carrijo Coube, 14-01, 17033-360 Bauru, SP, Brazil

Received 21 January 2014; Accepted 3 April 2014; Published 27 April 2014

Academic Editor: Chong-Zhi Wang

Copyright © 2014 Priscilla Maria Ponce Vareda et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Species of Myrcia are used by indigenous people and in traditional communities in Brazil for the treatment of Diabetes mellitus. We investigated the hypoglycemic effect of the extract of leaves of Myrcia bella in diabetic mice. The chemical fingerprinting of the 70% EtOH extract characterized as main constituents flavonoid aglycones, flavonoid-O-glycosides, and acylated flavonoid-O-glycosides derivatives of quercetin and myricetin. Mice were treated with saline or extract of M. bella (300 or 600 mg/Kg b.w.) for 14 days. Body weight and water and food intake were measured every day. Fasting blood glucose was measured weekly. At the end of the treatment, blood insulin, triglycerides, total cholesterol, and protein were measured. Glycogen content and expression of proteins of the insulin signaling pathway were measured in liver. The treatment with 600 mg/Kg reduced the fasting blood glucose in diabetic mice of the 7th day as water and food intake and increased hepatic glycogen. Total cholesterol and triglycerides were reduced in diabetic treated mice. The treatment increased the expression of IRS-1, PI3-K, and AKT in the livers of diabetic treated mice. The results indicate that the extract of the leaves of Myrcia bella has hypoglycemic properties and possibly acts to regulate glucose uptake by the liver.